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normal human breast epithelial cell line mcf 10a  (ATCC)


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    ATCC normal human breast epithelial cell line mcf 10a
    IC 50 values for the treated MDA-MB-231 cancer and <t> MCF-10A </t> normal cells
    Normal Human Breast Epithelial Cell Line Mcf 10a, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 8206 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/human+normal+breast+epithelial+cells+mcf+10a/MCF+10A/pmc13679565-81-12-19
    Average 99 stars, based on 8206 article reviews
    normal human breast epithelial cell line mcf 10a - by Bioz Stars, 2026-11
    99/100 stars

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    1) Product Images from "Mononuclear Mg( ii ) and Ca( ii ) metallocarboxylates of N -phthaloyl- γ -aminobutyric acid: synthesis, characterization, ds-DNA binding, preliminary anticancer evaluation against MDA-MB-231 cells, and computational studies"

    Article Title: Mononuclear Mg( ii ) and Ca( ii ) metallocarboxylates of N -phthaloyl- γ -aminobutyric acid: synthesis, characterization, ds-DNA binding, preliminary anticancer evaluation against MDA-MB-231 cells, and computational studies

    Journal: RSC Advances

    doi: 10.1039/d6ra07684j

    IC 50 values for the treated MDA-MB-231 cancer and MCF-10A normal cells
    Figure Legend Snippet: IC 50 values for the treated MDA-MB-231 cancer and MCF-10A normal cells

    Techniques Used:

    Related Articles

    Multiple Displacement Amplification:

    Article Title: TMED3 Promotes Proliferation and Migration in Breast Cancer Cells by Activating Wnt/β-Catenin Signaling
    Article Snippet: .. Breast cancer cell lines MCF-7, T47D, MDA-MB-231 and human normal breast epithelial cells MCF-10A (American Type Culture Collection, Manassas, VA, USA) were cultured in Dulbecco’s Modified Eagle Medium (DMEM) (Hyclone, USA) with 10% fetal bovine serum (FBS) (Hyclone, USA), 100IU/mL penicillin, and 100ug/mL streptomycin in a humidified cell incubator at 37°C and 5% CO 2 . .. TMED3 plasmid and siRNA sequences were provided by Genepharma Co., Ltd. (Shanghai, China).

    Article Title: CircWHSC1 Promotes Breast Cancer Progression by Regulating the FASN/AMPK/mTOR Axis Through Sponging miR-195-5p
    Article Snippet: .. Human normal breast epithelial cells MCF-10A and BC cell lines (MCF7, BT474, SKBr-3, ZR-75-30, MDA-MB-453 and MDA-MB-231) were bought from ATCC (Rockville, USA). .. These cells were grown in the RPMI-1640 complete medium (Thermo Scientific Hyclone, Utah, USA) which contained 10% fetal bovine serum (FBS, Thermo Scientific Hyclone, Utah, USA) and 1% penicillin/streptomycin (Yeasen Biotech Co., Ltd., Shanghai, China) and kept at 37°C with 5% CO 2 and saturated humidity.

    Article Title: <p>TMED3 Promotes Proliferation and Migration in Breast Cancer Cells by Activating Wnt/β-Catenin Signaling</p>
    Article Snippet: .. Cell Culture and Transfection Procedure Breast cancer cell lines MCF-7, T47D, MDA-MB-231 and human normal breast epithelial cells MCF-10A (American Type Culture Collection, Manassas, VA, USA) were cultured in Dulbecco’s Modified Eagle Medium (DMEM) (Hyclone, USA) with 10% fetal bovine serum (FBS) submit your manuscript | www.dovepress.com DovePress OncoTargets and Therapy 2020:135820 O nc oT ar ge ts a nd T he ra py d ow nl oa de d fr om h ttp s: //w w w .d ov ep re ss .c om / b y 18 5. ..

    Cell Culture:

    Article Title: TMED3 Promotes Proliferation and Migration in Breast Cancer Cells by Activating Wnt/β-Catenin Signaling
    Article Snippet: .. Breast cancer cell lines MCF-7, T47D, MDA-MB-231 and human normal breast epithelial cells MCF-10A (American Type Culture Collection, Manassas, VA, USA) were cultured in Dulbecco’s Modified Eagle Medium (DMEM) (Hyclone, USA) with 10% fetal bovine serum (FBS) (Hyclone, USA), 100IU/mL penicillin, and 100ug/mL streptomycin in a humidified cell incubator at 37°C and 5% CO 2 . .. TMED3 plasmid and siRNA sequences were provided by Genepharma Co., Ltd. (Shanghai, China).

    Article Title: <p>TMED3 Promotes Proliferation and Migration in Breast Cancer Cells by Activating Wnt/β-Catenin Signaling</p>
    Article Snippet: .. Cell Culture and Transfection Procedure Breast cancer cell lines MCF-7, T47D, MDA-MB-231 and human normal breast epithelial cells MCF-10A (American Type Culture Collection, Manassas, VA, USA) were cultured in Dulbecco’s Modified Eagle Medium (DMEM) (Hyclone, USA) with 10% fetal bovine serum (FBS) submit your manuscript | www.dovepress.com DovePress OncoTargets and Therapy 2020:135820 O nc oT ar ge ts a nd T he ra py d ow nl oa de d fr om h ttp s: //w w w .d ov ep re ss .c om / b y 18 5. ..

    Modification:

    Article Title: TMED3 Promotes Proliferation and Migration in Breast Cancer Cells by Activating Wnt/β-Catenin Signaling
    Article Snippet: .. Breast cancer cell lines MCF-7, T47D, MDA-MB-231 and human normal breast epithelial cells MCF-10A (American Type Culture Collection, Manassas, VA, USA) were cultured in Dulbecco’s Modified Eagle Medium (DMEM) (Hyclone, USA) with 10% fetal bovine serum (FBS) (Hyclone, USA), 100IU/mL penicillin, and 100ug/mL streptomycin in a humidified cell incubator at 37°C and 5% CO 2 . .. TMED3 plasmid and siRNA sequences were provided by Genepharma Co., Ltd. (Shanghai, China).

    Article Title: <p>TMED3 Promotes Proliferation and Migration in Breast Cancer Cells by Activating Wnt/β-Catenin Signaling</p>
    Article Snippet: .. Cell Culture and Transfection Procedure Breast cancer cell lines MCF-7, T47D, MDA-MB-231 and human normal breast epithelial cells MCF-10A (American Type Culture Collection, Manassas, VA, USA) were cultured in Dulbecco’s Modified Eagle Medium (DMEM) (Hyclone, USA) with 10% fetal bovine serum (FBS) submit your manuscript | www.dovepress.com DovePress OncoTargets and Therapy 2020:135820 O nc oT ar ge ts a nd T he ra py d ow nl oa de d fr om h ttp s: //w w w .d ov ep re ss .c om / b y 18 5. ..

    Transfection:

    Article Title: <p>TMED3 Promotes Proliferation and Migration in Breast Cancer Cells by Activating Wnt/β-Catenin Signaling</p>
    Article Snippet: .. Cell Culture and Transfection Procedure Breast cancer cell lines MCF-7, T47D, MDA-MB-231 and human normal breast epithelial cells MCF-10A (American Type Culture Collection, Manassas, VA, USA) were cultured in Dulbecco’s Modified Eagle Medium (DMEM) (Hyclone, USA) with 10% fetal bovine serum (FBS) submit your manuscript | www.dovepress.com DovePress OncoTargets and Therapy 2020:135820 O nc oT ar ge ts a nd T he ra py d ow nl oa de d fr om h ttp s: //w w w .d ov ep re ss .c om / b y 18 5. ..

    Control:

    Article Title: Synthesis and in vitro anticancer activity of ferrocenyl-aminoquinoline-carboxamide conjugates
    Article Snippet: 0020-1693/$ see front matter 2012 Elsevier B.V. A http://dx.doi.org/10.1016/j.ica.2012.06.039 ⇑ Corresponding author.. Tel.. : +1 604 822 4449; fax E-mail address: orvig@chem.ubc.ca (C. Orvig).



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    Figure 6. STAT3 phosphorylation had an inverse effect on CREB3L1 protein expression in TNBC. (a) Western blot analysis comparing the expression of STAT3, pSTAT3, and CREB3L1 in TNBC cells treated with scRNA to the same cell lines with ROR1 expression knocked down (siROR1). (b) Quantification of Western blot analysis (n = 3). Statistical significance was determined through paired Student’s t-test (* p < 0.05, ** p < 0.01). (c) Western blot analysis comparing the protein expression of ROR1, STAT3, pSTAT3, DNMT3A, DNMT3B, and CREB3L1 in naive <t>MCF10A</t> cells compared to the same cell line that has been transfected with IL-6 recombinant protein (* p < 0.05, ** p < 0.01, n = 3).
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    IC 50 values for the treated MDA-MB-231 cancer and MCF-10A normal cells

    Journal: RSC Advances

    Article Title: Mononuclear Mg( ii ) and Ca( ii ) metallocarboxylates of N -phthaloyl- γ -aminobutyric acid: synthesis, characterization, ds-DNA binding, preliminary anticancer evaluation against MDA-MB-231 cells, and computational studies

    doi: 10.1039/d6ra07684j

    Figure Lengend Snippet: IC 50 values for the treated MDA-MB-231 cancer and MCF-10A normal cells

    Article Snippet: The triple-negative breast cancer (TNBC) cell line MDA-MB-231 (ATCC HTB-26) and the normal human breast epithelial cell line MCF-10A (ATCC CRL-10317) used in this study were purchased from the American Type Culture Collection (ATCC), USA.

    Techniques:

    Figure 6. STAT3 phosphorylation had an inverse effect on CREB3L1 protein expression in TNBC. (a) Western blot analysis comparing the expression of STAT3, pSTAT3, and CREB3L1 in TNBC cells treated with scRNA to the same cell lines with ROR1 expression knocked down (siROR1). (b) Quantification of Western blot analysis (n = 3). Statistical significance was determined through paired Student’s t-test (* p < 0.05, ** p < 0.01). (c) Western blot analysis comparing the protein expression of ROR1, STAT3, pSTAT3, DNMT3A, DNMT3B, and CREB3L1 in naive MCF10A cells compared to the same cell line that has been transfected with IL-6 recombinant protein (* p < 0.05, ** p < 0.01, n = 3).

    Journal: Biomolecules

    Article Title: Uncovering a Novel Role of ROR1 in the Epigenetic Regulation of Tumor Suppressor Gene CREB3L1 in Triple-Negative Breast Cancer Cells.

    doi: 10.3390/biom15050734

    Figure Lengend Snippet: Figure 6. STAT3 phosphorylation had an inverse effect on CREB3L1 protein expression in TNBC. (a) Western blot analysis comparing the expression of STAT3, pSTAT3, and CREB3L1 in TNBC cells treated with scRNA to the same cell lines with ROR1 expression knocked down (siROR1). (b) Quantification of Western blot analysis (n = 3). Statistical significance was determined through paired Student’s t-test (* p < 0.05, ** p < 0.01). (c) Western blot analysis comparing the protein expression of ROR1, STAT3, pSTAT3, DNMT3A, DNMT3B, and CREB3L1 in naive MCF10A cells compared to the same cell line that has been transfected with IL-6 recombinant protein (* p < 0.05, ** p < 0.01, n = 3).

    Article Snippet: Cell Culture TNBC cell lines MDA-MB-231 (HTB 26, ATCC, Manassas, VA, USA), HCC1806 (CRL2335, ATCC, Manassas, VA, USA), and normal human epithelial breast cell line MCF10A (CRL-10317, ATCC, Manassas, VA, USA) are cultured as recommended by ATCC.

    Techniques: Phospho-proteomics, Expressing, Western Blot, Transfection, Recombinant